Recombinant CD34 Antibody (Biotin) - Primary antibody, specific to Human CD34

Cat. No.: Ab214215

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

P18K41

Conjugation

Biotin

Overview

Recombinant CD34 Antibody, clone P18K41, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant CD34 Antibody (Biotin) - Primary antibody, specific to Human CD34

Synonyms

CD34 antibody | CD34 antigen antibody | CD34 molecule antibody | CD34_HUMAN antibody | Cluster designation 34 antibody | cluster of differentiation 34 antibody | Hematopoietic progenitor cell antigen CD34 antibody | HPCA1 antibody | Mucosialin antibody |

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing

Host species

Mouse

Specificity

Human CD34

Immunogen

Tissue, cells or virus corresponding to Mouse CD34. Specifically, the murine endothelioma cell line tEnd.

Positive Control

Flow Cytometry: KG-1a cells

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD34 Antibody (Biotin) (Ab214215)

Recombinant CD34 Antibody (Biotin) (Ab214215) - Flow Cytometry
Flow cytometry analysis of CD34 expression by KG-1a cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD34 Antibody (Biotin) (Ab214215) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD34 Tbio Hematopoietic progenitor cell antigen CD34 (0 Activities)

Discover Target: CD34

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)