Recombinant CD8a Antibody (Biotin) - Primary antibody, specific to Human CD8a

Cat. No.: Ab214441

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

P20C02

Conjugation

Biotin

Overview

Recombinant CD8a Antibody, clone P20C02, could be used for Flow Cytometry

Specifications

Product Name

Recombinant CD8a Antibody (Biotin) - Primary antibody, specific to Human CD8a

Synonyms

alpha polypeptide (p32) antibody | CD_antigen=CD8a antibody | CD8 antibody | CD8 antigen alpha polypeptide antibody | CD8 antigen alpha polypeptide (p32) antibody | CD8 antigen, alpha polypeptide (p32) antibody | CD8a antibody | CD8A antigen antibody | CD

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com

Host species

Mouse

Specificity

Human CD8a

Immunogen

Human Peripheral Blood T Cells.

Positive Control

Flow Cytometry: Human peripheral blood lymphocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD8a Antibody (Biotin) (Ab214441)

Recombinant CD8a Antibody (Biotin) (Ab214441) - Flow Cytometry
Flow cytometry analysis of CD8a expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8a Antibody (Biotin) (Ab214441) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: Recombinant CD8a Antibody (Biotin) (Ab214441)

Recombinant CD8a Antibody (Biotin) (Ab214441) - Flow Cytometry
Flow cytometry analysis of CD8a expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8a Antibody (Biotin) (Ab214441) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)

Discover Target: CD8A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)