GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.See COA
ExactAb™, Validated, See COA ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human UBE2M/UBC12 Antibody, Monoclonal (OTI2D9), could be used for WB, IF/ICC and so on.
Specifications
Product Name
UBE2M/UBC12 Mouse mAb - Primary antibody, specific to UBE2M/UBC12, Rabbit IgG
Synonyms
hUbc12 antibody | hUbc12 antibody | NEDD8 carrier protein antibody | NEDD8 carrier protein antibody | NEDD8 conjugating enzyme Ubc12 antibody | NEDD8 conjugating enzyme Ubc12 antibody | NEDD8 protein ligase antibody | NEDD8 protein ligase antibody | NEDD8
Specifications & Purity
ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates speci
Host species
Mouse
Specificity
UBE2M/UBC12
Immunogen
Recombinant full length protein of human UBE2M produced in HEK293T.
Positive Control
WB: Hep G2, HeLa, A549, HEK293, Ramos, NIH/3T3 and C6 cell lysates. IF/ICC: COS7 cells transfected by pCMV6-ENTRY UBE2M.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
UBE2M/UBC12 Mouse mAb (Ab133254) - Western Blot All lanes: UBE2M/UBC12 Mouse mAb (Ab133254) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 20.0 s
UBE2M/UBC12 Mouse mAb (Ab133254) - IF/ICC UBE2M/UBC12 Mouse mAb (Ab133254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.
Application
Application
Dilution info
WB
1/500-1/2000
IF/ICC
1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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