DBC-1 Mouse mAb - Primary antibody, specific to CCAR2, Mouse IgG2a

Cat. No.: Ab099205

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IP, WB

Host species

Mouse

Reactive species

Human, Monkey, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

3G4-D11-D7

Conjugation

Unconjugated

Overview

Mouse anti Human DBC-1 Antibody, Monoclonal(3G4-D11-D7), could be used for WB, IF, ICC and so on .
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
IP: 1/20
Protein Function
Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.

Specifications

Product Name

DBC-1 Mouse mAb - Primary antibody, specific to CCAR2, Mouse IgG2a

Synonyms

DBC1 | DBC-1 | NET35 | p30DBC | p30 DBC | KIAA1967 | CCAR2 antibody | Cell cycle and apoptosis regulator 2 antibody | Cell division cycle and apoptosis regulator protein 2 antibody | DBC-1 antibody | DBC.1 antibody | DBC1 antibody | DBIRD complex subunit

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Host species

Mouse

Specificity

CCAR2

Immunogen

Recombinant protein of human DBC1 (AA 1-50)

Positive Control

WB: HeLa, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. IP: HeLa cells.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: DBC-1 Mouse mAb (Ab099205)

DBC-1 Mouse mAb (Ab099205) - Western Blot
All lanes: DBC-1 Mouse mAb (Ab099205) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 103 kDa
Observed band size: 126 kDa
Exposure time: 10.0 s

IF/ICC: DBC-1 Mouse mAb (Ab099205)

DBC-1 Mouse mAb (Ab099205) - IF/ICC
Immunocytochemistry analysis of DBC-1 in HeLa cells using DBC-1 Mouse mAb (Ab099205).

IP: DBC-1 Mouse mAb (Ab099205)

DBC-1 Mouse mAb (Ab099205) - IP
Immunoprecipitation analysis of DBC-1 in HeLa lysates using DBC-1 Mouse mAb (Ab099205).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IF/ICC:

  1/50-1/200 ;

• IP:

  1/20;

Associated Targets(Human)

CCAR2 Tbio Cell cycle and apoptosis regulator protein 2 (0 Activities)

Discover Target: CCAR2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)