GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Application
Capture Antibody, CLIA, Detection Antibody, ELISA, FIA, GICA, LF
Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human S100B Antibody, Monoclonal (5H2-3), is a Detection or Capture antibody could be used for ELISA, CLIA, LF, GICA, FIA. CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100µg/4µg AE FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
Specifications
Product Name
S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1
Synonyms
S100 calcium-binding protein B | S-100 protein beta chain | S-100 protein subunit beta
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA
26.0 kDa (Light Chain) & 46.1 kDa (Heavy Chain), under reducing conditions; 178.7 kDa, under non-reducing conditions.
Purification Method
Affinity chromatography
Matched Pairs
[Ab008407] Monoclonal S100B Antibody as Capture or Detection antibody in ELISA, CLIA, LF, GICA, FIA
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 37°C short term (7 days). Store at 4°C short term (30 days). Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Sandwich ELISA - S100B protein standard curve S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Sensitivity: 30.3 pg/mL Range: 0.1 ng/mL - 3.2 ng/mL
Sandwich ELISA - S100B protein standard curve S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148). Sensitivity: 18.8 pg/mL Range: 0.13 ng/mL - 3.1 ng/mL
Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA ·Capture antibody: S100B Mouse mAb (Ab008408) ·Detection antibody: S100B Mouse mAb (Ab008407)
Application
Application
Dilution info
CLIA
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
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