GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Carrier Free, ExactAb™, Validated, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human UBE2Z Antibody, Monoclonal (K52034_14G2), could be used for WB, IHC, IP and so on. Application: WB: 1/1000 IHC: 1/200 IP: 1/100 Protein Function: Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBE1L2, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation.
Specifications
Product Name
UBE2Z Mouse mAb - Primary antibody, specific to UBE2Z, Mouse IgG2a
WB: MCF7, Hep G2, HeLa, K562, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. IP: MCF7 lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
UBE2Z Mouse mAb (Ab133272) - Western Blot All lanes: UBE2Z Mouse mAb (Ab133272) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 41 kDa Exposure time: 13.3 s
UBE2Z Mouse mAb (Ab133272) - IP Immunoprecipitation was performed by incubation of 2.5 µg of UBE2Z Mouse mAb (Ab133272) with MCF7 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. UBE2Z Mouse mAb (Ab133272) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: MCF7 lysateLane 2: UBE2Z immunoprecipitated from MCF7 lysate by UBE2Z Mouse mAb (Ab133272) Lane 3: The isotype control antibody instead of UBE2Z Mouse mAb (Ab133272) in MCF7 lysate.Result: UBE2Z Mouse mAb (Ab133272) can immunoprecipitate UBE2Z.
UBE2Z Mouse mAb (Ab133272) - IHC IHC analysis of testis tissue by UBE2Z Mouse mAb (Ab133272).IHC was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with UBE2Z Mouse mAb (Ab133272) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the cytoplasm and nuclei.
Application
Application
Dilution info
WB
1/1000
IP
1/100
IHC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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