RPA70 Mouse mAb - Primary antibody, specific to RPA1, Mouse IgG2a

Cat. No.: Ab125738
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0 mg/mL
Application
IF/ICC, IP, WB
Host species
Mouse
Reactive species
Human, Monkey, Mouse, Rat
Isotype
Mouse IgG2a
Clone Number
8C3-D12-H10
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab125738-10μl
1
$99.90
50μl
Ab125738-50μl
1
$259.90
100μl
Ab125738-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab125738-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human RPA70 Antibody,  Monoclonal (8C3-D12-H10), could be used for WB, IF/ICC, IP and so on.

Application:
WB: 1/500-1/1000

IF/ICC: 1/50-1/200

IP: 1/20

Protein Function:

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.

Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Specifications

Product Name
RPA70 Mouse mAb - Primary antibody, specific to RPA1, Mouse IgG2a
Synonyms
MST075 | PFBMFT6 | REPA1 | RF-A | RP-A | RPA70 | Dmrpa1 antibody | Drosophila Replication Protein A antibody | DRPA antibody | HSSB antibody | Human single stranded DNA binding protein antibody | MST075 antibody | MSTP075 antibody | HSSB | p70 antibody |
Specifications & Purity
ExactAb™, Validated, 1.0 mg/mL
Host species
Mouse
Specificity
RPA1
Immunogen
Recombinant protein of human RPA70 (AA 317-616)
Positive Control
WB: HeLa and HEK293 cell lysates. IF/ICC: HeLa cells. IP: HeLa lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Mouse IgG2a
Purification Method
Protein G purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

RPA70 Mouse mAb (Ab125738) - Western Blot
All lanes: RPA70 Mouse mAb (Ab125738) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 66 kDa
Exposure time: 20.0 s

RPA70 Mouse mAb (Ab125738) - IF/ICC
Immunocytochemistry analysis of RPA70 in HeLa using RPA70 Mouse mAb (Ab125738).

RPA70 Mouse mAb (Ab125738) - IP
Immunoprecipitation analysis of RPA70 in HeLa lysates using RPA70 Mouse mAb (Ab125738).

Application
ApplicationDilution info
IF/ICC

1/50-1/200

IP

1/20

WB

1/500-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0809545Certificate of AnalysisMay 22, 2026 Ab125738
ZJ24F0809546Certificate of AnalysisMay 22, 2026 Ab125738
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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