GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human NAGK Antibody, Monoclonal (K1E005_8C10), could be used for WB, IHC, IP and so on. Application: WB: 1/1000 IHC: 1/50-1/250 IP: 1/100 Protein Function Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
Specifications
Product Name
NAGK Mouse mAb - Primary antibody, specific to NAGK, Mouse IgG1
WB: HeLa, Jurkat, HEK293, A431 and C6 cell lysates. IHC: Human tonsil tissue. IP: HeLa lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Stability And Storage
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
NAGK Mouse mAb (Ab117026) - Western Blot All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 11.4 s
NAGK Mouse mAb (Ab117026) - Western Blot All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 80.6 s
NAGK Mouse mAb (Ab117026) - IHC IHC analysis of tonsil tissue by NAGK Mouse mAb (Ab117026).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NAGK Mouse mAb (Ab117026) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm.
NAGK Mouse mAb (Ab117026) - IP Immunoprecipitation was performed by incubation of 2.5 µg NAGK Mouse mAb (Ab117026) with HeLa lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NAGK Mouse mAb (Ab117026) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.Lane 1: HeLa lysateLane 2: NAGK immunoprecipitated from HeLa lysate by NAGK Mouse mAb (Ab117026) Lane 3: The isotype control antibody instead of NAGK Mouse mAb (Ab117026) in HeLa lysate.Result: NAGK Mouse mAb (Ab117026) can immunoprecipitate NAGK.
Application
Application
Dilution info
WB
1/1000
IP
1/100
IHC
1/50-1/250
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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