GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human Alpha smooth muscle Actin Antibody, Monoclonal (F1), could be used for WB, IHC and so on. Application WB:0.001-2 μg/mL IHC: 5-20 µg/mL Protein Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Specifications
Product Name
alpha-Smooth Muscle Actin Mouse mAb - Primary antibody, specific to Alpha smooth muscle Actin, Mouse IgG1
Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species
Mouse
Specificity
Alpha smooth muscle Actin
Immunogen
Recombinant ACTA2 expressed in E.coli (AA 167-377).
Positive Control
WB: NIH/3T3, Mouse skeletal and Mouse heart lysates; IHC: Human Ovarian cancer tissues, Human Rectum tissue, Rat Skeletal muscle tissues, Rat Lung tissue, Mouse Pancreas tissues, Mouse Spleen tissues.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 5.6 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - Western Blot All lanes: Alpha smooth muscle Actin Mouse mAb (Ab088443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 26.7 s
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Ovarian cancer Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Human Rectum Tissue Primary Ab:10ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Skeletal muscle Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Rat Lung Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Pancreas Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Alpha smooth muscle Actin Mouse mAb (Ab088443) - IHC DAB staining on IHC Sample: Mouse Spleen Tissue Primary Ab: 3ug/mL Alpha smooth muscle Actin Mouse mAb (Ab088443) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Application
Application
Dilution info
IHC
5-20 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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