BRAF Mouse mAb - Primary antibody, specific to BRAF, Mouse IgG1

Cat. No.: Ab091608
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
IF/ICC, WB
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
125CT13.3.5
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab091608-10μl
4
$79.90
50μl
Ab091608-50μl
2
$259.90
100μl
Ab091608-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab091608-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human BRAF Antibody, Monoclonal (125CT13.3.5), could be used for WB, IF, ICC and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/10-1/50
Protein Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Specifications

Product Name
BRAF Mouse mAb - Primary antibody, specific to BRAF, Mouse IgG1
Synonyms
FLJ95109 antibody | 94 kDa B raf protein antibody | B raf 1 antibody | B raf antibody | B Raf proto oncogene serine threonine protein kinase antibody | B Raf proto oncogene, serine/threonine kinase antibody | B RAF1 antibody | B-Raf proto-oncogene serine/
Specifications & Purity
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species
Mouse
Specificity
BRAF
Immunogen
BRAF recombinant protein (AA 4-365).
Positive Control
WB: MCF-7, K562, HeLa, Hep G2 and HEK293 cell lysates. ICC/IF: C2C12 cells
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

BRAF Mouse mAb (Ab091608) - Western Blot
All lanes: BRAF Mouse mAb (Ab091608) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 85 kDa
Exposure time: 17.9 s

BRAF Mouse mAb (Ab091608) - ICC/IF
Fluorescent image of C2C12 cells stained with BRAF Antibody (Ab091608). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody 1:25, 1 h at 37℃. For secondary antibody, AF488 conjugated donkey anti-mouse antibody (green) was used 1:400, 50 min at 37℃. Cytoplasmic actin was counterstained with AF555 (red) conjugated Phalloidin 7 units/mL, 1 h at 37℃. BRAF immunoreactivity is localized to Cytoplasm significantly.

Application
ApplicationDilution info
WB

1/500-1/2000

ICC/IF

1/10-1/50

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
BRAF Tclin Serine/threonine-protein kinase B-raf (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
BRAF Tclin Serine/threonine-protein kinase B-raf (11587 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
BRAF Tclin Protein cereblon/BRAF (4 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F0800966Certificate of AnalysisSep 04, 2023 Ab091608
ZJ23F0800965Certificate of AnalysisSep 04, 2023 Ab091608
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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