MUC1/CD227 Mouse mAb (AF647) - Primary antibody, specific to MUC1/CD227

Cat. No.: Ab213577

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG3

Clone Number

C595 (NCRC48)

Conjugation

AF647

Overview

Mouse anti Human MUC1/CD227 Antibody, Monoclonal (C595 (NCRC48)), could be used for Flow Cytometry.

Specifications

Product Name

MUC1/CD227 Mouse mAb (AF647) - Primary antibody, specific to MUC1/CD227

Synonyms

MCD | PUM | MCKD | MCKD1 | ADMCKD | ADTKD2 | Ca15-3 | ADMCKD1 | CA 15-3 | MUC-1/X | MUC-1/SEC | Breast carcinoma-associated antigen DF3 | Carcinoma-associated mucin | CD227 antigen | CD227 | DF3 antigen | EMA | Episialin | H23 antigen | H23AG | KL-6 | MAM

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is invol

Host species

Mouse

Specificity

MUC1/CD227

Immunogen

Purified human MUC1

Positive Control

Flow Cytometry: MCF-7

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: MUC1/CD227 Mouse mAb (AF647) (Ab213577)

MUC1/CD227 Mouse mAb (AF647) (Ab213577) - Flow Cytometry
Flow cytometry analysis of MUC1/CD227 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with MUC1/CD227 Mouse mAb (AF647) (Ab213577) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

MUC1 Tchem Mucin-1 (0 Activities)

Discover Target: MUC1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)