beta Tubulin Mouse mAb - Primary antibody, specific to beta tubulin, IgG3

Cat. No.: Ab179921
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. 1.0mg/mL
Application
Flow Cytometry, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG3
Clone Number
AB01/1E7
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab179921-10μl
1

$45.90

$66.90
Save $21.00 (31.39%)
20μl
Ab179921-20μl
3
$99.90
50μl
Ab179921-50μl
4

$137.90

$199.90
Save $62.00 (31.02%)
100μl
Ab179921-100μl
2
$299.90
1ml
Ab179921-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,499.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
WB: 1/2,000-1/10,0000; Predicted molecular weight: 50 kDa
Flow: 0.002 μg/mL - 0.25 μg/mL
Protein Function:
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Specifications

Product Name
beta Tubulin Mouse mAb - Primary antibody, specific to beta tubulin, IgG3
Synonyms
Beta 4 tubulin antibody | Beta 5 tubulin antibody | beta Ib tubulin antibody | Beta1 tubulin antibody | Class I beta tubulin antibody | M40 antibody | MGC117247antibody | MGC16435 antibody | OK/SW cl.56 antibody | OK/SWcl.56 antibody | TBB5_HUMAN antibody
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0mg/mL
Host species
Mouse
Specificity
beta tubulin
Immunogen
Recombinant human beta tubulin
Positive Control
WB: HeLa, A431, HEK293, MCF7, NIH3T3 and C6 whole cell lysates Flow: HeLa cells
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
IgG3
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
1.0mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

beta Tubulin Mouse mAb (Ab179921) - Western Blot
All lanes: beta Tubulin Antibody (Ab179921) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 50.0 kDa
Exposure time: 90.0 s 

beta Tubulin Mouse mAb (Ab179921) - Flow Cytometry
Overlay histogram showing HeLa cells stained with beta Tubulin Mouse mAb (Ab179921) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Application
ApplicationDilution info
WB

1/2,000-1/10,0000; Predicted molecular weight: 50 kDa

Flow Cytometry

0.002 μg/mL - 0.25 μg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
TUBB Tclin Tubulin beta chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202057Certificate of AnalysisDec 13, 2023 Ab179921
ZJ23F1202058Certificate of AnalysisDec 13, 2023 Ab179921
ZJ23F1202059Certificate of AnalysisDec 13, 2023 Ab179921
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →

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