c-Fos Mouse mAb - Primary antibody, specific to c-Fos, Mouse IgG1

Cat. No.: Ab096159

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

WB

Host species

Mouse

Reactive species

Human, Monkey, Rat

Isotype

Mouse IgG1

Clone Number

7D6-A9-D4

Conjugation

Unconjugated

Overview

Mouse anti Human c-Fos Antibody, Monoclonal (7D6-A9-D4), could be used for WB and so on.

Application:

WB: 1/500-1/1000

Protein Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

Specifications

Product Name

c-Fos Mouse mAb - Primary antibody, specific to c-Fos, Mouse IgG1

Synonyms

Cellular oncogene fos | G0/G1 switch regulatory protein 7

Specifications & Purity

ExactAb™, Validated, 1.0 mg/mL

Host species

Mouse

Specificity

c-Fos

Immunogen

Purified recombinant human FOS protein fragments expressed in E.coli (AA 1-380).

Positive Control

WB: HeLa (serum starved 36 hours, then treated with 20% FBS for 2 hours) cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: c-Fos Mouse mAb (Ab096159)

c-Fos Mouse mAb (Ab096159) - Western Blot
All lanes: c-Fos Mouse mAb (Ab096159) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 67 kDa
Exposure time: 91.4 s

Application

• Application: Dilution info
• WB:

  1/500-1/1000 ;

Associated Targets(Human)

FOS Tbio Proto-oncogene c-Fos (0 Activities)

Discover Target: FOS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action