Glycophorin A/CD235a Mouse mAb - Primary antibody, specific to Glycophorin A/CD235a, Mouse IgG1

Cat. No.: Ab105788

GRADE & PURITY Animal Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Blocking, Flow Cytometry, Functional Assay

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

6A7M

Conjugation

Unconjugated

Overview

Mouse anti Human Glycophorin A/CD235a Antibody, Monoclonal (6A7M), could be used for Flow, in vitro GPA blockade and so on.

Application:
Flow: Use at an assay dependent concentration.

in vitro GPA blockade: Use at an assay dependent concentration.

Protein Function
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

Specifications

Product Name

Glycophorin A/CD235a Mouse mAb - Primary antibody, specific to Glycophorin A/CD235a, Mouse IgG1

Synonyms

MN | CD235a antigen | CD235a | glycophorin A (includes MN blood group) | glycophorin A (MNS blood group) | Glycophorin A | glycophorin Erik | glycophorin MiI | glycophorin MiIII | glycophorin MiV | glycophorin MiX | glycophorin SAT | glycophorin Sta type

Specifications & Purity

Animal Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Mouse

Specificity

Glycophorin A/CD235a

Immunogen

Type O, Rh- human erythrocytes and purified glycophorin A

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

27.3 kDa (Light Chain) & 52.8 kDa (Heavy Chain), under reducing conditions; 208.8 kDa, under non-reducing conditions.

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: Glycophorin A/CD235a Mouse mAb (Ab105788)

Glycophorin A/CD235a Mouse mAb (Ab105788) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling Glycophorin A/CD235a (red) with Glycophorin A/CD235a Mouse mAb (Ab105788) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  Use at an assay dependent concentration.

• in vitro GPA blockade:

  Use at an assay dependent concentration;