MHC class II I E kappa Mouse mAb (FITC) - Primary antibody, specific to MHC class II I E kappa

Cat. No.: Ab210122
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:498nm ? Fluorescence excitation maximum at 498 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:517nm ? Fluorescence emission maximum at 517 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human, Rat
Isotype
Mouse IgG2a
Clone Number
14-4-4S
Conjugation
FITC
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab210122-10μg
4
$39.90
25μg
Ab210122-25μg
3
$81.90
100μg
Ab210122-100μg
1
$219.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL Em:517nm,Ex:498nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Mouse/Rat MHC class II I E kappa Antibody, Monoclonal (14-4-4S), could be used for Flow Cytometry.

Specifications

Product Name
MHC class II I E kappa Mouse mAb (FITC) - Primary antibody, specific to MHC class II I E kappa
Synonyms
Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | H2 Ea antibody | H2-Ea antibody
Specifications & Purity
ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL
Host species
Mouse
Specificity
MHC class II I E kappa
Immunogen
C3H mouse skin graft and spleen cells
Positive Control
Flow: rat splenocytes
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
Em:517nm, Ex:498nm, ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

MHC class II I E kappa Mouse mAb (FITC) (Ab210122) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC class II I E kappa Mouse mAb (FITC) (Ab210122) at 0.1μg/test for 1 hour at 4°C. Left panel – Mouse IgG (FITC) (Ab180307).

MHC class II I E kappa Mouse mAb (FITC) (Ab210122) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC class II I E kappa Mouse mAb (FITC) (Ab210122) at 0.1μg/test for 1 hour at 4°C. Blue - Mouse IgG (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
HLA-DRB5 Tbio HLA class II histocompatibility antigen, DR beta 5 chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F1216371Certificate of AnalysisJul 10, 2026 Ab210122
ZJ24F1216372Certificate of AnalysisJul 10, 2026 Ab210122
ZJ24F1216373Certificate of AnalysisJul 10, 2026 Ab210122
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:498nm grade guide → View Em:517nm grade guide →

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