Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
WB: 1/1,000 - 1/20,000; Predicted molecular weight: 42.0 KDa
Flow: 1/200 - 1/50,000
ICC/IF: 1/1,000
alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Western Blot
All lanes: alpha-Smooth Muscle Actin Mouse Antibody (Ab187009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 43 kDa
Exposure time: 60.0 s
alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Western Blot
All lanes: alpha-Smooth Muscle Actin Mouse Antibody (Ab187009) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 54.9 s
alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Western Blot
All lanes: alpha-Smooth Muscle Actin Mouse Antibody (Ab187009) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 60.0 s
alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with alpha-Smooth Muscle Actin Mouse mAb (Ab187009) (Red) at 0.2 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with alpha-Smooth Muscle Actin Mouse mAb (Ab187009) (Red) at 0.2 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
alpha-Smooth Muscle Actin Mouse mAb (Ab187009) - ICC/IF
IF analysis of alpha-Smooth Muscle Actin (Red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha-Smooth Muscle Actin Mouse mAb (Ab187009) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (Cy5) (Ab179004) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1/200 - 1/50,000 |
| WB | 1/1,000 - 1/20,000; Predicted molecular weight: 42.0 KDa |
| IF/ICC | 1/1000 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708535 | Certificate of Analysis | May 21, 2026 | Ab187009 |
| ZJ24F0708536 | Certificate of Analysis | May 21, 2026 | Ab187009 |
| ZJ24F0708537 | Certificate of Analysis | May 21, 2026 | Ab187009 |
| ZJ26F0636104 | Certificate of Analysis | May 21, 2026 | Ab187009 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
