MHC class I/H-2Kb Mouse mAb - Primary antibody, specific to MHC class I/H-2Kb, Mouse IgG2b

Cat. No.: Ab115272

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, Purification

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2b

Clone Number

Y-3

Conjugation

Unconjugated

Overview

Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (Y-3), could be used for in vivo MHC II blockade, Functional assays, Purification of MHC peptide complexes, Flow and so on.
Application:
in vivo MHC II blockade: Use at an assay dependent concentration.
Functional assays: Use at an assay dependent concentration.
Purification of MHC peptide complexes: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.

Specifications

Product Name

MHC class I/H-2Kb Mouse mAb - Primary antibody, specific to MHC class I/H-2Kb, Mouse IgG2b

Synonyms

K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Host species

Mouse

Specificity

MHC class I/H-2Kb

Immunogen

Con A stimulated spleen cells from BALB.B mice

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: MHC class I/H-2Kb Rat mAb (Ab115272)

MHC class I/H-2Kb Rat mAb (Ab115272) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling MHC class I/H-2Kb with MHC class I/H-2Kb Rat mAb (Ab115272) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: MHC class I/H-2Kb Rat mAb (Ab115272)

MHC class I/H-2Kb Rat mAb (Ab115272) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling MHC class I/H-2Kb (red) with MHC class I/H-2Kb Rat mAb (Ab115272) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Functional Assay:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo MHC II blockade:

  Use at an assay dependent concentration;

• Purification of MHC peptide complexes:

  Use at an assay dependent concentration.;