alpha Tubulin Mouse mAb - Primary antibody, specific to alpha tubulin, IgG2b

Cat. No.: Ab179938

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, 1.0 mg/mL

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2b

Clone Number

AB01/5A12

Conjugation

Unconjugated

Overview

Application
WB: 1/1,000-1/20,000; Predicted molecular weight: 50 kDa
Flow: 0.032 -1.0 μg/mL
ICC/IF: 2.0 μg/mL

Specifications

Product Name

alpha Tubulin Mouse mAb - Primary antibody, specific to alpha tubulin, IgG2b

Synonyms

Alpha tubulin ubiquitous antibody | Alpha-tubulin ubiquitous antibody | K alpha 1 antibody | TBA1B_HUMAN antibody | TUBA1B antibody | Tubulin alpha 1B antibody | Tubulin alpha 1B chain antibody | Tubulin alpha ubiquitous antibody | Tubulin alpha ubiquitou

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL

Host species

Mouse

Specificity

alpha tubulin

Immunogen

Recombinant human alpha tubulin

Positive Control

WB: HeLa, MCF-7, Jurkat, HepG2, A549, Raji, HEK293, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

IgG2b

Light Chain Type

kappa

SDS-PAGE

185 kDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Western Blot: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - Western Blot
All lanes: alpha Tubulin Antibody (Ab179938) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 50.0 kDa
Exposure time: 8.8 s 

Flow Cytometry: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - Flow Cytometry
Overlay histogram showing HeLa cells stained with alpha Tubulin Mouse mAb (Ab179938) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).

ICC/IF: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - ICC/IF
IF analysis of alpha Tubulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - ICC/IF
IF analysis of alpha Tubulin (Green) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

ICC/IF: alpha Tubulin Mouse mAb (Ab179938)

alpha Tubulin Mouse mAb (Ab179938) - ICC/IF
IF analysis of alpha Tubulin (Red) in U2OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with alpha Tubulin Mouse mAb (Ab179938) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179006) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• IF/ICC:

  2 μg/mL;

• Flow Cytometry:

  0.032 μg/mL - 1.0 μg/mL;

• WB:

  1/1,000-1/20,000; Predicted molecular weight: 50 kDa;

Associated Targets(Human)

TUBA1B Tchem Tubulin alpha-1B chain (0 Activities)

Discover Target: TUBA1B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action