GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1mg/mL
Application
IHC, WB
Host species
Mouse
Reactive species
Cow, Dog, Goat, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep
ExactAb™, Validated, Carrier Free, 1mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse monoclonal (C209) to ATP1A1 could be uesd for WB, IHC and so on. Application: Western blotting: 0.5-2µg/mL Immunohistochemistry: 5-20µg/mL Protein Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Post-translational Phosphorylation on Tyr-10 modulates pumping activity.
Specifications
Product Name
ATP1A1 Mouse mAb - Primary antibody, specific to ATP1A1, IgG2b
Recombinant ATP1a1 (His493~Ala660) expressed in E.coli
Positive Control
WB: HEK293, Hep G2 and C6 cell lysates. IHC: Human Placenta Tissue, Human Liver cancer Tissue, Human Kidney Tissue, Porcine Kidney Tissue, Rat Uterus Tissue, Rat Small intestine Tissue.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
IgG2b
Light Chain Type
kappa
SDS-PAGE
150kDa
Purification Method
Protein A + Protein G affinity chromatography
Concentration
1mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
ATP1A1 Mouse mAb (Ab088306) - Western Blot All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 100 kDa Observed band size: 95 kDa Exposure time: 117.2 s
ATP1A1 Mouse mAb (Ab088306) - Western Blot All lanes: ATP1A1 Mouse mAb (Ab088306) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 100 kDa Observed band size: 95 kDa Exposure time: 30.0 s
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Placenta Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Liver cancer Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 10 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Porcine Kidney Tissue Primary Ab: 20 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Rat Uterus Tissue Primary Ab: 3 µg/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
ATP1A1 Mouse mAb (Ab088306) - IHC DAB staining on IHC Sample: Rat Small intestine Tissue Primary Ab: 3 ug/mL ATP1A1 Mouse mAb (Ab088306) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Application
Application
Dilution info
WB
1/1000 - 1/2000
WB
0.5-2µg/mL
IHC
5-20µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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