MHC class I Mouse mAb (AF647) - Primary antibody, specific to MHC class I

Cat. No.: Ab189656

GRADE & PURITY Validated, ExactAb™, Ex:650nm, Em:668nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG2a

Clone Number

34-1-2S

Conjugation

AF647

Overview

Mouse anti Mouse MHC class I Antibody, Monoclonal (34-1-2S), could be used for Flow, in vivo activation of APCs and so on.

Specifications

Product Name

MHC class I Mouse mAb (AF647) - Primary antibody, specific to MHC class I

Synonyms

H-2D | H2-D | H2-K1 | K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alph

Specifications & Purity

ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL

Host species

Mouse

Specificity

MHC class I

Immunogen

BDF mouse spleen cells

Positive Control

Flow: C57BL/6 mouse splenocytes.

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

Em:668nm, Ex:650nm, ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: MHC class I Mouse mAb (AF647) (Ab189656)

MHC class I Mouse mAb (AF647) (Ab189656) - Flow Cytometry
Flow cytometry analysis of MHC class I expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I Mouse mAb (AF647) (Ab189656) at 0.2μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF647) (Ab190077). 

Flow Cytometry: MHC class I Mouse mAb (AF647) (Ab189656)

MHC class I Mouse mAb (AF647) (Ab189656) - Flow Cytometry
Flow cytometry analysis of MHC class I expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I Mouse mAb (AF647) (Ab189656) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume ;

Associated Targets(Human)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)