MHC class II I E kappa Mouse mAb (PE) - Primary antibody, specific to MHC class II I E kappa

Cat. No.: Ab210123

GRADE & PURITY Validated, ExactAb™, Ex:565nm, Em:575nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

14-4-4S

Conjugation

PE

Overview

Mouse anti Mouse/Rat MHC class II I E kappa Antibody, Monoclonal (14-4-4S), could be used for Flow Cytometry.

Specifications

Product Name

MHC class II I E kappa Mouse mAb (PE) - Primary antibody, specific to MHC class II I E kappa

Synonyms

Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | H2 Ea antibody | H2-Ea antibody

Specifications & Purity

ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL

Host species

Mouse

Specificity

MHC class II I E kappa

Immunogen

C3H mouse skin graft and spleen cells

Positive Control

Flow: rat splenocytes

Conjugation

PE

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grade

Em:575nm, Ex:565nm, ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do n

Images

Flow Cytometry: MHC class II I E kappa Mouse mAb (PE) (Ab210123)

MHC class II I E kappa Mouse mAb (PE) (Ab210123) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with MHC class II I E kappa Mouse mAb (PE) (Ab210123) at 0.3μg/test for 1 hour at 4°C. Left panel – Mouse IgG (PE) (Ab180308).

Flow Cytometry: MHC class II I E kappa Mouse mAb (PE) (Ab210123)

MHC class II I E kappa Mouse mAb (PE) (Ab210123) - Flow Cytometry
Flow cytometry analysis of MHC class II I E kappa expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with MHC class II I E kappa Mouse mAb (PE) (Ab210123) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume. ;

Associated Targets(Human)

HLA-DRB5 Tbio HLA class II histocompatibility antigen, DR beta 5 chain (0 Activities)

Discover Target: HLA-DRB5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)