H-2Kb bound to SIINFEKL Mouse mAb (FITC) - Primary antibody, specific to H-2Kb bound to SIINFEKL

Cat. No.: Ab209993

GRADE & PURITY Validated, ExactAb™, Ex:498nm, Em:517nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG1

Clone Number

25-D1.16

Conjugation

FITC

Overview

Mouse anti Mouse H-2Kb bound to SIINFEKL Antibody, Monoclonal (25-D1.16), could be used for Flow.

Specifications

Product Name

H-2Kb bound to SIINFEKL Mouse mAb (FITC) - Primary antibody, specific to H-2Kb bound to SIINFEKL

Synonyms

A 28 antibody | A 9 antibody | Antigen presenting molecule antibody | Aw 24 antibody | Aw 68 antibody | CLASS I HISTOCOMPATIBILITY ANTIGEN antibody | H2 K1 antibody | H2K antibody | HLA A antibody | HLA class I histocompatibility antigen A 1 alpha chain a

Specifications & Purity

ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL

Host species

Mouse

Specificity

H-2Kb bound to SIINFEKL

Immunogen

SIINFEKL pulsed RMA-S cells

Positive Control

Flow: C57BL/6 mouse splenocytes​

Conjugation

FITC

Excitation (nm)

Blue Laser(488nm)

Emission(nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grade

Em:517nm, Ex:498nm, ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: H-2Kb bound to SIINFEKL Mouse mAb (FITC) (Ab209993)

H-2Kb bound to SIINFEKL Mouse mAb (FITC) (Ab209993) - Flow Cytometry
C57BL/6 mouse splenocytes cells treated with 30μM OVA Peptide for 2 hours (right panel) were stained with H-2Kb bound to SIINFEKL Mouse mAb (FITC) (Ab209993) (red) at 0.1μg/test for 1 hour at 4°C. Blue - Mouse IgG (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody. Untreated spleen cells were used as control (left panel).

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test. For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

HLA-A Tbio HLA class I histocompatibility antigen, A-69 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)