OBFC1 Mouse mAb - Primary antibody, specific to STN1

Cat. No.: Ab119117

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

OTI2E4

Conjugation

Unconjugated

Overview

Mouse anti Human OBFC1 Antibody, Monoclonal (OTI2E4), could be used for WB, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

OBFC1 Mouse mAb - Primary antibody, specific to STN1

Synonyms

AAF 44 | AAF44 | Alpha accessory factor 44 | bA541N10.2 | CST complex subunit STN1 | FLJ22559 | Obfc1 | Oligonucleotide/oligosaccharide binding fold containing 1 | Oligonucleotide/oligosaccharide-binding fold-containing protein 1 | OTTHUMP00000020417 | OT

Specifications & Purity

ExactAb™, Validated, See COA

Biochemical and Physiological Mechanisms

Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bin

Host species

Mouse

Specificity

STN1

Immunogen

Recombinant protein of human OBFC1.

Positive Control

WB: Hep G2, A549, Jurkat and MCF7 celllysates. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY OBFC. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY OBFC1.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - Western Blot
All lanes: OBFC1 Mouse mAb (Ab119117) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 100.0 s

IF/ICC: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - IF/ICC
OBFC1 Mouse mAb (Ab119117) immunofluorescent staining of COS7cells transiently transfected by pCMV6-ENTRYOBFC1.

Flow Cytometry​: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - Flow Cytometry​
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by OBFC1 Mouse mAb (Ab119117) , and then analyzed by flow cytometry.

Application

• Application: Dilution info
• WB:

  1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

Associated Targets(Human)

STN1 Tbio CST complex subunit STN1 (0 Activities)

Discover Target: STN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)