beta Actin Mouse mAb (HRP) - Primary antibody, specific to beta actin, IgG1

Cat. No.: Ab175849
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. 0.5 mg/mL
Application
WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
FG01/2D10
Conjugation
HRP
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab175849-10μl
≥10
$66.90
20μl
Ab175849-20μl
≥10
$99.90
50μl
Ab175849-50μl
10

$159.90

$199.90
Save $40.00 (20.01%)
100μl
Ab175849-100μl
2
$299.90
1ml
Ab175849-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,499.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, High Performance, Validated, Azide Free, 0.5 mg/mL Azide Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application:
WB :1:2000-1:10000

Specifications

Product Name
beta Actin Mouse mAb (HRP) - Primary antibody, specific to beta actin, IgG1
Synonyms
A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal
Specifications & Purity
ExactAb™, High Performance, Validated, Azide Free, 0.5 mg/mL
Host species
Mouse
Specificity
beta actin
Immunogen
Synthetic peptide in beta Actin
Positive Control
WB: K562, A549, HeLa, Jurkat, NIH3T3 and C6 whole cell lysates
Conjugation
HRP
Grade
Azide Free, ExactAb™, High Performance, Validated
Isotype
IgG1
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.
Images

beta Actin Mouse mAb (HRP) (Ab175849) - Western Blot
All lanes: beta Actin Mouse mAb (HRP) (Ab175849) at 1/5000 dilution
Samples: Lysates at 20 µg per lane

Predicted band size: 42.0 kDa
Observed band size: 42.0 kDa
Exposure time: 41.8s

Application
ApplicationDilution info
WB

1/2000 - 1/10000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
ACTB Tbio Actin, cytoplasmic 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202286Certificate of AnalysisMar 17, 2026 Ab175849
ZJ23F1202287Certificate of AnalysisMar 17, 2026 Ab175849
ZJ23F1202288Certificate of AnalysisMar 17, 2026 Ab175849
ZJ23F1202289Certificate of AnalysisMar 17, 2026 Ab175849
ZJ23R1200202Certificate of AnalysisDec 21, 2023 Ab175849
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide →

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