CD74 Mouse mAb (Biotin) - Primary antibody, specific to CD74, Mouse IgG1

Cat. No.: Ab185159

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

LN2

Conjugation

Biotin

Overview

Flow: ≤1.0 μg/test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

CD74 Mouse mAb (Biotin) - Primary antibody, specific to CD74, Mouse IgG1

Synonyms

CD74 Monoclonal Antibody (LN2), Biotin | Biotin Anti-Human CD74 Antibody[LN2] | Biotin Mouse Anti-Human CD74 | Biotin anti-human CD74 Antibody | Protein 41 antibody | p35 antibody | p33 antibody | MHC HLA-DR gamma chain antibody | MHC HLA DR gamma chain a

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Mouse

Specificity

CD74

Immunogen

SU-DHL-4 cells.

Positive Control

Flow: Human peripheral blood lymphocytes, Raji

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD74 Mouse mAb (Biotin) (Ab185159)

CD74 Mouse mAb (Biotin) (Ab185159) - Flow Cytometry
Flow cytometry analysis of CD74 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD74 Mouse mAb (Biotin) (Ab185159) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: CD74 Mouse mAb (Biotin) (Ab185159)

CD74 Mouse mAb (Biotin) (Ab185159) - Flow Cytometry
Flow cytometry analysis of CD74 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD74 Mouse mAb (Biotin) (Ab185159) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD74 Mouse mAb (Biotin) (Ab185159)

CD74 Mouse mAb (Biotin) (Ab185159) - Flow Cytometry
Flow cytometry analysis of CD74 expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with CD74 Mouse mAb (Biotin) (Ab185159) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  5μl/test

  For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Associated Targets(Human)

CD74 Tchem HLA class II histocompatibility antigen gamma chain (0 Activities)

Discover Target: CD74

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD74 Tchem HLA-DR antigens-associated invariant chain (25 Activities)

Discover Target: CD74

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action