Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Application
WB: 1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa
Flow: 0.02 μg/mL - 2.5 μg/mL
HMGB1 Mouse mAb (Ab156561) - Western Blot
All lanes: HMGB1 Antibody (Ab156561) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 24.9 kDa
Observed band size: 27.2 kDa
Exposure time: 118.1 s
HMGB1 Mouse mAb (Ab156561) - Western Blot
All lanes: HMGB1 Antibody (Ab156561) at 0.1 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 24.9 kDa
Observed band size: 26.1 kDa
Exposure time: 27.8 s
HMGB1 Mouse mAb (Ab156561) - ICC/IF
IF analysis of HMGB1 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HMGB1 Mouse mAb (Ab156561) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| WB | 1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa |
| Flow | 0.02 μg/mL – 2.5 μg/mL |
| WB | 1/2,000 - 1/50,000; Predicted molecular weight: 25.0 kDa |
| WB | 1/2,000 - 1/100,000; Predicted molecular weight: 25.0 kDa |
| Flow Cytometry | 0.02 μg/mL - 2.5 μg/mL |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303593 | Certificate of Analysis | Feb 10, 2026 | Ab156561 |
| ZJ24F0303594 | Certificate of Analysis | Feb 10, 2026 | Ab156561 |
| ZJ24F0303595 | Certificate of Analysis | Feb 10, 2026 | Ab156561 |
| ZJ24F0303596 | Certificate of Analysis | Feb 10, 2026 | Ab156561 |
| ZJ24R0200300 | Certificate of Analysis | Mar 01, 2024 | Ab156561 |
| ZJ23F1202037 | Certificate of Analysis | Dec 12, 2023 | Ab156561 |
| ZJ23F1202036 | Certificate of Analysis | Dec 12, 2023 | Ab156561 |
| ZJ23F1202035 | Certificate of Analysis | Dec 12, 2023 | Ab156561 |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
