Recombinant CD19 Antibody (Biotin) - Primary antibody, specific to CD19

Cat. No.: Ab213314

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

P03D32

Conjugation

Biotin

Overview

Recombinant CD19 Antibody, clone P03D32, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

Recombinant CD19 Antibody (Biotin) - Primary antibody, specific to CD19

Synonyms

Antibody deficiency due to defect in CD19 antibody | Antibody deficiency due to defect in CD19, included antibody | AW495831 antibody | B lymphocyte antigen CD19 antibody | B lymphocyte surface antigen B4 antibody | B-lymphocyte antigen CD19 antibody | B-

Specifications & Purity

Validated, ExactAb™, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes (PubMed:29523808). Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16

Host species

Mouse

Specificity

CD19

Positive Control

Flow Cytometry: Human peripheral blood lymphocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD19 Antibody (Biotin) (Ab213314)

Recombinant CD19 Antibody (Biotin) (Ab213314) - Flow Cytometry
Flow cytometry analysis of CD19 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab213314) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: Recombinant CD19 Antibody (Biotin) (Ab213314)

Recombinant CD19 Antibody (Biotin) (Ab213314) - Flow Cytometry
Flow cytometry analysis of CD19 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab213314) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD19 Tclin B-lymphocyte antigen CD19 (0 Activities)

Discover Target: CD19

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)