Recombinant HLA-DR Antibody - Primary antibody, specific to Human HLA-DR

Cat. No.: Ab219607

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE)

Application

ELISA, Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

P22B24

Conjugation

Unconjugated

Overview

Recombinant HLA-DR Antibody, clone P22B24, could be used for Flow Cytometry, ELISA and so on.

Specifications

Product Name

Recombinant HLA-DR Antibody - Primary antibody, specific to Human HLA-DR

Synonyms

FLJ51114 | histocompatibility antigen HLA-DR alpha | HLA class II histocompatibility antigen, DR alpha chain | HLADR | HLA-DR | HLA-DRA | HLA-DRA1 | major histocompatibility complex, class II, DR alpha | MHC cell surface glycoprotein | MHC class II antige

Specifications & Purity

Animal Free, Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE)

Biochemical and Physiological Mechanisms

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell recep

Host species

Mouse

Specificity

Human HLA-DR

Immunogen

Human lymphoblastoid B-cell line RPMI 8866

Positive Control

Flow Cytometry: Human peripheral blood lymphocytes; Raji cells

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Recombinant, Validated

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: Recombinant HLA-DR Antibody (Ab219607)

Recombinant HLA-DR Antibody (Ab219607) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphcytes labelling HLA-DR with Recombinant HLA-DR Antibody (Ab219607) (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Ab170221) (left panel) and detected with AF 488-conjugated affinipure goat anti-Mouse IgG.

Flow Cytometry: Recombinant HLA-DR Antibody (Ab219607)

Recombinant HLA-DR Antibody (Ab219607) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphcytes labelling HLA-DR (red) with Recombinant HLA-DR Antibody (Ab219607) at 0.5 μg/mL for 1 hour at 4°C, and detected with AF 488-conjugated affinipure goat anti-Mouse IgG. Blue - Mouse IgG Isotype Control Antibody (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant HLA-DR Antibody (Ab219607)

Recombinant HLA-DR Antibody (Ab219607) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling HLA-DR (red) with Recombinant HLA-DR Antibody (Ab219607) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

SEC: Recombinant HLA-DR Antibody (Ab219607)

Recombinant HLA-DR Antibody (Ab219607) - SEC
The purity of Recombinant HLA-DR Antibody (Ab219607) is more than 95% verified by HPLC.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5 µg per million cells in 100 µl volume;

Associated Targets(Human)

HLA-DRA Tbio HLA class II histocompatibility antigen, DR alpha chain (0 Activities)

Discover Target: HLA-DRA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)