HLA-DR Mouse mAb - Primary antibody, specific to HLA-DR, Mouse IgG2a

Cat. No.: Ab108247

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, See COA

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

L243

Conjugation

Unconjugated

Overview

Mouse anti Human HLA-DR Antibody, Monoclonal (L243), could be used for Flow and so on.
Application:
Flow: 0.06 μg/100w cells
Protein Function
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Specifications

Product Name

HLA-DR Mouse mAb - Primary antibody, specific to HLA-DR, Mouse IgG2a

Synonyms

FLJ51114 | histocompatibility antigen HLA-DR alpha | HLA class II histocompatibility antigen, DR alpha chain | HLADR | HLA-DR | HLA-DRA | HLA-DRA1 | major histocompatibility complex, class II, DR alpha | MHC cell surface glycoprotein | MHC class II antige

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Host species

Mouse

Specificity

HLA-DR

Immunogen

Human lymphoblastoid B-cell line RPMI-8866

Positive Control

Flow: Human peripheral blood lymphocytes.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: HLA-DR Mouse mAb (Ab108247)

HLA-DR Mouse mAb (Ab108247) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA-DR with HLA-DR Mouse mAb (Ab108247) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: HLA-DR Mouse mAb (Ab108247)

HLA-DR Mouse mAb (Ab108247) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA-DR (red) with HLA-DR Mouse mAb (Ab108247) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: HLA-DR Mouse mAb (Ab108247)

HLA-DR Mouse mAb (Ab108247) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling HLA-DR (red) with HLA-DR Mouse mAb (Ab108247) at 0.1 μg/mL for 1 hour at 4°C. AffiniPure Goat anti-Mouse IgG, Fcγ fragment specific (AF647) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  0.06 μg/100w cells;

Associated Targets(Human)

HLA-DRA Tbio HLA class II histocompatibility antigen, DR alpha chain (0 Activities)

Discover Target: HLA-DRA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)