Survivin Mouse mAb - Primary antibody, specific to Survivin, Mouse IgG1

Cat. No.: Ab170500

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, IF/ICC

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

CD01/1H8

Conjugation

Unconjugated

Overview

Application
Flow: 1/200 - 1/12,000
ICC/IF: 1/200

Specifications

Product Name

Survivin Mouse mAb - Primary antibody, specific to Survivin, Mouse IgG1

Synonyms

Apoptosis inhibitor 4 antibody | Apoptosis inhibitor survivin antibody | Apoptosis inhibitor4 antibody | Baculoviral IAP repeat containing 5 antibody | Baculoviral IAP repeat containing protein 5 antibody | Baculoviral IAP repeat-containing protein 5 anti

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Host species

Mouse

Specificity

Survivin

Immunogen

Recombinant full length protein corresponding to Human Survivin

Positive Control

Flow: HeLa cells ICC/IF: U-2 OS cells

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150.0 KDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Flow Cytometry: Survivin Mouse mAb (Ab170500)

Survivin Mouse mAb (Ab170500) - Flow Cytometry
Flow Cytometry analysis of HeLa cells labelling Survivin (Red) with Survivin Mouse mAb (Ab170500) at 5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

ICC/IF: Survivin Mouse mAb (Ab170500)

Survivin Mouse mAb (Ab170500) - ICC/IF
IF analysis of Survivin (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Survivin Mouse mAb (Ab170500) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• IF/ICC:

  1/200 ;

• Flow:

  1/200 - 1/12,000;

Associated Targets(Human)

BIRC5 Tchem Baculoviral IAP repeat-containing protein 5 (0 Activities)

Discover Target: BIRC5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action