Transferrin Receptor/CD71 Mouse mAb (AF647) - Primary antibody, specific to Transferrin Receptor/CD71

Cat. No.: Ab210004

GRADE & PURITY Validated, ExactAb™, Ex:650nm, Em:668nm, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

OKT-9

Conjugation

AF647

Overview

Mouse anti Human Transferrin Receptor/CD71 Antibody, Monoclonal (OKT-9), could be used for Flow.

Specifications

Product Name

Transferrin Receptor/CD71 Mouse mAb (AF647) - Primary antibody, specific to Transferrin Receptor/CD71

Synonyms

TFR | TRFR | IMD46 | CD71 antigen | CD71 | CD71TRFR | p90 | T9 | TfR (Transferrin R) | TfR (TransferrinR) | TFR1 | TFRC | TR | transferrin receptor (p90, CD71) | transferrin receptor protein 1 | CD 71 antibody | CD71 antibody | CD71 antigen antibody | IMD

Specifications & Purity

ExactAb™, Validated, Ex:650nm, Em:668nm, 5 μL/test

Biochemical and Physiological Mechanisms

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface wi

Host species

Mouse

Specificity

Transferrin Receptor/CD71

Immunogen

Human acute lymphoblastic leukemia cells

Positive Control

Flow: Jurkat

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

Em:668nm, Ex:650nm, ExactAb™, Validated

Purification Method

Protein G purified

Concentration

5 μL/test

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Transferrin Receptor/CD71 Mouse mAb (AF647) (Ab210004)

Transferrin Receptor/CD71 Mouse mAb (AF647) (Ab210004) - Flow Cytometry
Flow cytometry analysis of Transferrin Receptor/CD71 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with Transferrin Receptor/CD71 Mouse mAb (AF647) (Ab210004) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF647) (Ab190077). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  5 μL/test. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Associated Targets(Human)

TFRC Tclin Transferrin receptor protein 1 (0 Activities)

Discover Target: TFRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)