EnzymoPure™, BioReagent BioReagent,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 75 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Taxonomy Tree
| Kingdom | Organic compounds |
|---|---|
| Superclass | Organoheterocyclic compounds |
| Class | Lactams |
| Subclass | Beta lactams |
| Intermediate Tree Nodes | Not available |
| Direct Parent | Carbapenems |
| Alternative Parents | Benzyloxycarbonyls Alpha amino acids and derivatives Nitrobenzenes Pyrroline carboxylic acids Phenoxy compounds Phenol ethers Nitroaromatic compounds Monoalkyl phosphates Azepines Alkyl aryl ethers Vinylogous esters Tertiary carboxylic acid amides Enoate esters Secondary alcohols Azetidines Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Monocarboxylic acids and derivatives Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds |
| Molecular Framework | Aromatic heteropolycyclic compounds |
| Substituents | Carbapenem - Benzyloxycarbonyl - Alpha-amino acid or derivatives - Nitrobenzene - Phenoxy compound - Nitroaromatic compound - Pyrroline carboxylic acid or derivatives - Pyrroline carboxylic acid - Phenol ether - Monoalkyl phosphate - Azepine - Alkyl aryl ether - Benzenoid - Alkyl phosphate - Phosphoric acid ester - Organic phosphoric acid derivative - Monocyclic benzene moiety - Vinylogous ester - Alpha,beta-unsaturated carboxylic ester - Enoate ester - Tertiary carboxylic acid amide - Pyrroline - Organic nitro compound - Secondary alcohol - C-nitro compound - Carboxylic acid ester - Carboxamide group - Azetidine - Azacycle - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Organic oxoazanium - Monocarboxylic acid or derivatives - Ether - Carboxylic acid derivative - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Carbonyl group - Alcohol - Aromatic heteropolycyclic compound |
| Description | This compound belongs to the class of organic compounds known as carbapenems. These are beta-lactam derivatives in which the beta-lactam ring shares the nitrogen atom with a pyrrole-2-carboxylic acid. |
| External Descriptors | Not available |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 I2118428 | Certificate of Analysis | Apr 03, 2026 | P274341 |
| I2118430 | Certificate of Analysis | Apr 03, 2026 | P274341 |
| I2118431 | Certificate of Analysis | Apr 03, 2026 | P274341 |
| I2118432 | Certificate of Analysis | Apr 03, 2026 | P274341 |
| J2531104 | Certificate of Analysis | Nov 06, 2025 | P274341 |
| J2513042 | Certificate of Analysis | Oct 17, 2025 | P274341 |
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| 2. Kairui Duan, Nabila Mehwish, Mengdie Xu, Hu Zhu, Jiajun Hu, Mian Lin, Lu Yu, Bae Hoon Lee. (2023) Autoclavable Albumin-Based Cryogels with Uncompromising Properties. Gels, 9 (9): (712). [PMID:37754393] [10.3390/gels9090712] |
| 3. Jie Zhang, Zhe Kong, Xiangyao Li, Chuang Zhang, Kaijun Xu, Guiqing Cheng, Zhongwei Wang, Qing Yu. (2023) The influence of Scutellaria baicalensis extract obtained from traditional Chinese herbal medicine on the thermo-oxidative degradation and stabilization of plasticized polylactic acid. POLYMER ENGINEERING AND SCIENCE, 63 (9): (3105-3115). [PMID:] [10.1002/pen.26431] |
| 4. Zixuan Ding, Peilin Wang, Zhenrun Li, Yupeng Guo, Qiang Ma. (2023) Sulfur dots/Au@Ag nanorods array-based polarized ECL sensor for the detection of thyroid cancer biomarker. TALANTA, [PMID:37437396] [10.1016/j.talanta.2023.124925] |
| 5. Qingxu Song, Gongde Lan, Qian Li, Yang Lin, Xin Chen, Hong Liu, Yufeng Cheng, Yuxia Luan. (2023) A hydrogel-enabled cascade amplification of radiosensitization and immune activation for cancer radiotherapy. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2023.142201] |
| 6. Yanhua Xu, Huiyan Dong, Chenxi Liu, Haiwei Lou, Renyong Zhao. (2023) Efficient Aflatoxin B1 degradation by a novel isolate, Pseudomonas aeruginosa M-4. FOOD CONTROL, [PMID:] [10.1016/j.foodcont.2023.109679] |
| 7. Lihuang Wu, Yuqi Wang, Xinyue Zhao, Hongli Mao, Zhongwei Gu. (2023) Investigating the Biodegradation Mechanism of Poly(trimethylene carbonate): Macrophage-Mediated Erosion by Secreting Lipase. BIOMACROMOLECULES, [PMID:36644840] [10.1021/acs.biomac.2c01350] |
| 8. Guoli Xue, Yanjun Qu, Dan Wu, Shuyuan Huang, Yuqing Che, Jing Yu, Ping Song. (2023) Biodegradation of Aflatoxin B1 in the Baijiu Brewing Process by Bacillus cereus. Toxins, 15 (1): (65). [PMID:36668884] [10.3390/toxins15010065] |
| 9. Xiangpeng Han, Mengyu Zhang, Jiayi Peng, Jinsong Wu, Qingping Zhong. (2022) Purification and characterization of a novel bacteriocin from Lactiplantibacillus plantarum Z057, and its antibacterial and antibiofilm activities against Vibrio parahaemolyticus. LWT-FOOD SCIENCE AND TECHNOLOGY, [PMID:] [10.1016/j.lwt.2022.114358] |
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| 11. Mengdie Xu, Nabila Mehwish, Bae Hoon Lee. (2022) Facile Fabrication of Transparent and Opaque Albumin Methacryloyl Gels with Highly Improved Mechanical Properties and Controlled Pore Structures. Gels, 8 (6): (367). [PMID:35735711] [10.3390/gels8060367] |
| 12. Fangjun Liu, Dun Wang, Jiaqi Wang, Liwei Ma, Cuiyun Yu, Hua Wei. (2022) Construction of Enzyme-Responsive Micelles Based on Theranostic Zwitterionic Conjugated Bottlebrush Copolymers with Brush-on-Brush Architecture for Cell Imaging and Anticancer Drug Delivery. MOLECULES, 27 (9): (3016). [PMID:35566368] [10.3390/molecules27093016] |
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| 14. Xiaoping Wu, Qiujie Pang, Fuxiang Zhi, Xiaohong Mao, Yingbin Hu. (2022) Overexpression of long non-coding RNA ACTA2-AS1 inhibits the viability, proliferation, migration and invasion of colorectal cancer cells. TISSUE & CELL, [PMID:35325674] [10.1016/j.tice.2022.101769] |
| 15. Tao Zhang, Dekun Zhang, Hongtao Liu, Kai Chen. (2021) Quantitative analysis and degradation mechanisms of different protein degradation methods. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 110 (5): (1034-1043). [PMID:34842354] [10.1002/jbm.b.34977] |
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| 17. Subramani Kandhasamy, Bo Liang, Da-Peng Yang, Yiming Zeng. (2021) Antibacterial Vitamin K3 Carnosine Peptide-Laden Silk Fibroin Electrospun Fibers for Improvement of Skin Wound Healing in Diabetic Rats. ACS Applied Bio Materials, [PMID:35007027] [10.1021/acsabm.0c01650] |
| 18. Wei Yihua, Zhang Jinyan, Qiu Shuyan, Huang Qingqing, Yuan Linfeng, Chen Liumeng, Dai Tingcan, Tu Tianhua, Zhang Biaojin, Yan Han, Li Weihong. (2021) Selenium Species Determination in Se-Enriched Grain Crops with Foliar Spray of Sodium Selenite by IP-RP-HPLC-UV-HG-AFS. Food Analytical Methods, 14 (7): (1345-1358). [PMID:] [10.1007/s12161-021-01975-9] |
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| 24. Chao Wang, Xiaodan Jia, Wenyao Zhen, Mengchao Zhang, Xiue Jiang. (2019) Small-Sized MOF-Constructed Multifunctional Diagnosis and Therapy Platform for Tumor. ACS Biomaterials Science & Engineering, [PMID:33438409] [10.1021/acsbiomaterials.9b00813] |
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| 32. Ying-Hui Shan, Li-Hua Peng, Xin Liu, Xi Chen, Jie Xiong, Jian-Qing Gao. (2014) Silk fibroin/gelatin electrospun nanofibrous dressing functionalized with astragaloside IV induces healing and anti-scar effects on burn wound. INTERNATIONAL JOURNAL OF PHARMACEUTICS, [PMID:25556053] [10.1016/j.ijpharm.2014.12.067] |
| 33. Ziyu Gan, Jun Wang. (2025) A MOF-stabilized enzyme-based colorimetric biosensor integrated with pH test strips for portable detection of D-limonene in agricultural applications. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2025.161719] |
| 34. Wei Bao, Yunbao Gao, Jianing Zhang, Jing Jin, Na Zhang, Baijun Liu, Mingyao Zhang, Zhaohui Su, Hongwen Liang, Xiangling Ji, Wei Jiang. (2025) Biodegradable poly (lactic acid) blends toughened with in situ formed core-shell structure by reactive processing. POLYMER, [PMID:] [10.1016/j.polymer.2025.128330] |
| 35. Xue Yunfan, Li Xiaoyan, Fan Zhuizhui, Ji Yating, Wang Jie, Chen Qinhu, Cai Zaisheng. (2025) Eco-friendly Anti-felting Finishing and Low-Temperature Dyeing of Wool Through Plant Protease. FIBERS AND POLYMERS, [PMID:] [10.1007/s12221-024-00838-4] |
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| 39. Yu Zhengtao, Xia Ying, Li Jiameng, Jiang Junwen, Li You, Li Youjun, Wang Liu. (2024) METTL3 mediates m6A modification of lncRNA CRNDE to promote ATG10 expression and improve brain ischemia/reperfusion injury through YTHDC1. Biology Direct, 19 (1): (1-17). [PMID:39407279] [10.1186/s13062-024-00536-4] |
| 40. Jiajun Pan, Zhaopeng Xia, Haibao Zhang, Yang Lu, Yong Liu. (2024) One-step bleaching method for animal fiber by persulphate/hydrogen peroxide: Degradation mechanism of eumelanin. Journal of Cleaner Production, [PMID:] [10.1016/j.jclepro.2024.142256] |
| 41. Wenlong Pang, Bin Li, Yufeng Wu, Yu Zhang, Jun Yang, Zhongxun Tian, Shaonan Tian, Jianjun Li. (2024) One-Step Synthesis of PtPb Alloy Nanoparticles Via Wet Chemical Method for the Upgraded Recycling of PLA Plastic. ACS Sustainable Chemistry & Engineering, [PMID:] [10.1021/acssuschemeng.4c06032] |
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| 43. Ruopeng Yin, Tao Wang, Jingzu Sun, Huanqin Dai, Yuting Zhang, Ningning Liu, Hongwei Liu. (2024) Postbiotics From Lactobacillus Johnsonii Activates Gut Innate Immunity to Mitigate Alcohol-Associated Liver Disease. Advanced Science, [PMID:39574408] [10.1002/advs.202405781] |
| 44. Shitong Wang, Kai Huang, Wenjing Shi, Jiajia Mo, Honghui Shi, Chuntai Liu, Baobao Chang. (2024) Promoting the formation of stereocomplex crystal in PLLA/PDLA by crosslinking restricted phase separation and solid-state stretching induced orientation. POLYMER, [PMID:] [10.1016/j.polymer.2024.127670] |
| 45. Xiaofei Wang, Jili Han, Shuxian Zhang, Keshuai Liu, Xiaoxuan Fan, Changcai Bai, Guoning Chen. (2024) Self-polymerization silica nanoparticles based molecularly imprinted polymers for selective recognition of protein. JOURNAL OF CHROMATOGRAPHY A, [PMID:39142168] [10.1016/j.chroma.2024.465260] |
| 46. Kanzhen Liu, Cuiping Pang, Qinghua Li, Jianghua Li, Guocheng Du, Guoqiang Zhang. (2025) Step Enzymatic Hydrolysis and In Silico Screening-Assisted Preparation of Bioactive Peptides from Abalone. Foods, 14 (7): (1209). [PMID:40238341] [10.3390/foods14071209] |
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| 48. Shuming Liu, Chunhua Ge, Zhiqiang Li, Keke Chen, Xuefeng Li, Chengyao Peng, Xiangdong Zhang. (2025) High Thermal Conductivity and Biocompatibility Silk Fibroin Hydrogels Filled with CDs@BNOH for Body Surface Cooling. ACS Applied Materials & Interfaces, [PMID:40641287] [10.1021/acsami.5c09260] |
| 49. Mao Wenqiang, Li Jiapeng, Wang Dongdong, Pan Yanxiong, Ji Xiangling, Jiang Wei. (2025) Biodegradable CPPC/PBS/PLA blends with enhanced adhesive properties by in situ compatibilization. COLLOID AND POLYMER SCIENCE, [PMID:] [10.1007/s00396-025-05476-7] |
| 50. Yuchao Luo, Yingjie Hu, Tianyi Peng, Li Wen, Zikang Zhang, Ye Liu, Chengxin Chen, Yuxiang Wu, Zhihan Liu, Qingxia Liu, Zhixiang Chen. (2025) Self-charging, antibacterial, and biodegradable functional materials prepared by electrospinning: for masks, wound dressings, and vascular grafts. CHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.cej.2025.166917] |
| 51. Xiaohai Bu, Liyi Chai, Yanmei Liu, Bing Feng, Tao Lu, Mingxin Feng, Yuming Zhou. (2025) Asymmetric hierarchically structured composite aerogels with enhanced dual-spectral selectivity for efficient radiative cooling. COMPOSITES PART B-ENGINEERING, [PMID:] [10.1016/j.compositesb.2025.112988] |
| 52. Ranran Ma, Weiwei Peng, Yanli Xie, Nazish Muzaffar, Weibin Ma, Yuhui Yang, Qian Li, Hang Jia. (2025) Mechanistic Insights into Aflatoxin B1 and M1 Degradation by Bacillus subtilis HNGD-Mq02 from Wheat Koji and Its Application in Food Detoxification. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:40890944] [10.1021/acs.jafc.5c05089] |
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| 62. Yang Yang, Gengyu Zhou, Yang Chen, Ning Lin, Jingzhi Ma. (2025) Poly(lactide acid)-based microneedles enhanced by tunicate cellulose nanocrystals for potential diabetic periodontitis treatment. CARBOHYDRATE POLYMERS, [PMID:40368556] [10.1016/j.carbpol.2025.123629] |
| 63. Dandan Feng, Zhu Zhu, Shangpeng Wang, Yangcan Gao, Yingwen Li, Yuying Sun, Jiquan Zhang. (2025) A type I crustin with an inhibitory effect on proteases and strong binding capacity to chitin from Neocaridina denticulata sinensis. Comparative Immunology Reports, [PMID:] [10.1016/j.cirep.2025.200226] |
| 64. Jing Zhao, Maoning Li, Wenbang Liao, Yufei Shao, Qingyuan Du, Dandan Li, Yu Nan, Zhi Zhang, Dazhi Sun. (2025) Hierarchically porous degradable foam prepared by water-in-oil high internal phase emulsion template for high-performance recyclable oil - water separation. JOURNAL OF HAZARDOUS MATERIALS, [PMID:41207227] [10.1016/j.jhazmat.2025.140385] |
| 65. Ling Ma, Shu-Ling Yang, Wen-Qian Lian, Xin-Feng Wei, Kai Zhang, Rui-Ying Bao, Wei Yang. (2025) Encapsulation of Enzymes in Metal–Organic Frameworks with Dual Thermal Protection for Melt Processing of Self-Biodegradable Plastics. MACROMOLECULES, [PMID:] [10.1021/acs.macromol.5c00584] |
| 66. Ke Zhan, Yuting Zhao, Haiying Li, Shan Jiang. (2025) Octopod-shaped silver–gold alloy for NIR-driven synergistic photothermal and catalytic bacterial ablation. COLLOIDS AND SURFACES B-BIOINTERFACES, [PMID:41380240] [10.1016/j.colsurfb.2025.115353] |
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