Recombinant CD45 Antibody (AF405) - Primary antibody, specific to CD45

Cat. No.: Ab213119
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:401nm ? Fluorescence excitation maximum at 401 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:422nm ? Fluorescence emission maximum at 422 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 5 μL/test
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
P51B13
Conjugation
AF405
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10T
Ab213119-10T
≥10
$87.90
25T
Ab213119-25T
9
$173.90
100T
Ab213119-100T
8
$437.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Ex:401nm, Em:422nm, 5 μL/test Em:422nm,Ex:401nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant CD45 Antibody, clone P51B13, could be used for Flow Cytometry and so on.

Specifications

Product Name
Recombinant CD45 Antibody (AF405) - Primary antibody, specific to CD45
Synonyms
B220 | CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase | PTPRC | receptor-type tyrosine-protein phosphatase C | T200 Glycoprotein | T200 leukocyte common antigen | T200 | B220
Specifications & Purity
ExactAb™, Validated, Ex:401nm, Em:422nm, 5 μL/test
Biochemical and Physiological Mechanisms
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second
Host species
Mouse
Specificity
CD45
Immunogen
Peripheral blood mononuclear cells
Positive Control
Flow: Human peripheral blood lymphocytes
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
Em:422nm, Ex:401nm, ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Recombinant CD45 Antibody (AF405) (Ab213119) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant Recombinant CD45 Antibody (AF405) (Ab213119) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF405) (Ab190076).

Recombinant CD45 Antibody (AF405) (Ab213119) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (AF405) (Ab213119) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ24F1216377Certificate of AnalysisJul 10, 2026 Ab213119
ZJ24F1216378Certificate of AnalysisJul 10, 2026 Ab213119
ZJ24F1216379Certificate of AnalysisJul 10, 2026 Ab213119
ZJ24R1201601Certificate of AnalysisDec 21, 2024 Ab213119
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:401nm grade guide → View Em:422nm grade guide →

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