Recombinant CD45 Antibody (Biotin) - Primary antibody, specific to CD45

Cat. No.: Ab213127

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

P56D02

Conjugation

Biotin

Overview

Recombinant CD45 Antibody, clone P56D02, could be used for Flow Cytometry and so on.

Specifications

Product Name

Recombinant CD45 Antibody (Biotin) - Primary antibody, specific to CD45

Synonyms

B220 | CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase | PTPRC | receptor-type tyrosine-protein phosphatase C | T200 Glycoprotein | T200 leukocyte common antigen | T200 | B220

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second

Host species

Mouse

Specificity

CD45

Immunogen

Human PBMC

Positive Control

Flow: Human peripheral blood lymphocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant CD45 Antibody (Biotin) (Ab213127)

Recombinant CD45 Antibody (Biotin) (Ab213127) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213127) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: Recombinant CD45 Antibody (Biotin) (Ab213127)

Recombinant CD45 Antibody (Biotin) (Ab213127) - Flow Cytometry
Flow cytometry analysis of CD45 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (Biotin) (Ab213127) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)

Discover Target: PTPRC

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)