PD-L1 Mouse mAb - Primary antibody, specific to PD-L1, Mouse IgG2b

Cat. No.: Ab121011

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, See COA

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

29E.2A3

Conjugation

Unconjugated

Overview

Mouse anti Human PD-L1 Antibody, Monoclonal (29E.2A3), could be used for Flow and so on.
Application:
Flow: 0.06 μg/100w cells
Protein Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Specifications

Product Name

PD-L1 Mouse mAb - Primary antibody, specific to PD-L1, Mouse IgG2b

Synonyms

Avelumab | B7-H | B7H1 | B7-H1 | B7H1PDCD1L1 | CD274 antigenMGC142294 | CD274 molecule | CD274 | PDCD1L1 | PDCD1LG1 | PDCD1LG1MGC142296 | PDL1 | PD-L1 | PD-L1B7 homolog 1 | PDL1PDCD1 ligand 1 | programmed cell death 1 ligand 1 | Programmed death ligand 1

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Host species

Mouse

Specificity

PD-L1

Immunogen

Full length human PD-L1

Positive Control

Flow: Stimulate human PBMC with PHA

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Mouse IgG2b

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Shipped at 4°C. Store at 4°C long term (12 months). Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: PD-L1 Mouse mAb (Ab121011)

PD-L1 Mouse mAb (Ab121011) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 500 ng/mL Ionomycin (I139530) and 10 ng/mL PMA (P408905) co-stimulated (right) Jurkat cells labelling PD-L1 (red) with PD-L1 Mouse mAb (Ab121011) at 0.1 μg/mL for 1 hour at 4°C. Goat anti Mouse IgG H&L(PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  0.06 μg/100w cells;

Associated Targets(Human)

CD274 Tclin Programmed cell death 1 ligand 1 (0 Activities)

Discover Target: CD274

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action