NSE Mouse mAb - Primary antibody, specific to NSE, Mouse IgG2b

Cat. No.: Ab118776
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. See COA
Application
IF/ICC, IHC, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG2b
Clone Number
C10
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab118776-10μl
5
$79.90
50μl
Ab118776-50μl
5
$207.90
100μl
Ab118776-100μl
1
$411.90
1ml
Ab118776-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$2,799.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Carrier Free, ExactAb™, Validated, See COA Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human NSE Antibody, Monoclonal (C10), could be used for WB, IHC, ICC, IF and so on.
Application:
WB: 0.5-2µg/mL
IHC: 5-30µg/mL
ICC/IF: 5-30µg/mL
Protein Function
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Specifications

Product Name
NSE Mouse mAb - Primary antibody, specific to NSE, Mouse IgG2b
Synonyms
2-phospho-D-glycerate hydro-lyase | EC 4.2.1.11 | Enolase 2 | Neural enolase | Neuron-specific enolase | NSE
Specifications & Purity
Carrier Free, ExactAb™, Validated, See COA
Host species
Mouse
Specificity
NSE
Immunogen
Recombinant NSE expressed in E.coli (AA 2-285)
Positive Control
WB: Hep G2, NIH/3T3, C6 and Jurkat cell lysates. IHC: Human Glioma Tissue, Human Cerebrum Tissue. IF/ICC: Hep G2 cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG2b
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

NSE Mouse mAb (Ab118776) - Western Blot
All lanes: NSE Mouse mAb (Ab118776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 16.4 s

NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Glioma Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.


NSE Mouse mAb (Ab118776) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 1µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application
ApplicationDilution info
IHC

5-30 µg/mL

WB

0.5-2 µg/mL

IF/ICC

5-30 µg/mL

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
ENO2 Tbio Gamma-enolase (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0911401Certificate of AnalysisJul 14, 2026 Ab118776
ZJ23F0901047Certificate of AnalysisSep 18, 2023 Ab118776
ZJ23F0901046Certificate of AnalysisSep 18, 2023 Ab118776
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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