HLA Class I Mouse mAb - Primary antibody, specific to HLA Class I, Mouse IgG2a

Cat. No.: Ab108167

GRADE & PURITY Carrier Free, Validated, Low Endotoxin, Azide Free, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Application

Flow Cytometry, Functional Assay, IF/ICC, IHC, IP, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

W6/32

Conjugation

Unconjugated

Overview

Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for WB, Flow, IP, IHC, ICC/IF, Functional Assays and so on.
Application
ICC/IF: 5.0µg/mL
WB: 5.0µg/mL
Flow: 1.0-5.0µg/mL
IP: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Functional Assays: Use at an assay dependent concentration.

Specifications

Product Name

HLA Class I Mouse mAb - Primary antibody, specific to HLA Class I, Mouse IgG2a

Synonyms

A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I | HLAA | HLA-A | HLA-ABC | major histo

Specifications & Purity

Carrier Free, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Host species

Mouse

Specificity

HLA Class I

Immunogen

Human tonsil cell membrane

Positive Control

Flow Cyt: Jurkat cells. ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, Low Endotoxin, PBS Only, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: HLA Class I Mouse mAb (Ab108167)

HLA Class I Mouse mAb (Ab108167) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA Class I with HLA Class I Mouse mAb (Ab108167) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150). 

Flow Cytometry: HLA Class I Mouse mAb (Ab108167)

Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA Class I (red) with HLA Class I Mouse mAb (Ab108167) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• IF/ICC:

  5.0 μg/mL;

• Functional Assay:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• Flow:

  1-5 μg/mL;

• IHC:

  Use at an assay dependent concentration.;

• WB:

  5.0µg/mL;

Associated Targets(Human)

HLA-A Tbio HLA class I histocompatibility antigen, A-69 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-74 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-26 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-24 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-68 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-11 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-80 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-43 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-34 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-2 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-66 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-23 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-32 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-33 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-29 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-30 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-36 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tclin HLA class I histocompatibility antigen, A-3 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-1 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-25 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action