NKG2D Mouse mAb (Biotin) - Primary antibody, specific to NKG2D, Mouse IgG1

Cat. No.: Ab185179

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

1D11

Conjugation

Biotin

Overview

Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

NKG2D Mouse mAb (Biotin) - Primary antibody, specific to NKG2D, Mouse IgG1

Synonyms

CD314 antigen | CD314 | D12S2489E | FLJ17759 | FLJ75772 | Killer cell lectin-like receptor subfamily K member 1 | killer cell lectin-like receptor subfamily K, member 1 | KLR | KLRK1 | NK cell receptor D | NKG2-D type II integral membrane protein | NKG2D

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Host species

Mouse

Specificity

NKG2D

Immunogen

NKL cells

Positive Control

Flow: Human peripheral blood lymphocytes.

Conjugation

Biotin

Grade

ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: NKG2D Mouse mAb (Biotin) (Ab185179)

NKG2D Mouse mAb (Biotin) (Ab185179) - Flow Cytometry
Flow cytometry analysis of NKG2D expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with NKG2D Mouse mAb (Biotin) (Ab185179) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306).

Flow Cytometry: NKG2D Mouse mAb (Biotin) (Ab185179)

NKG2D Mouse mAb (Biotin) (Ab185179) - Flow Cytometry
Flow cytometry analysis of NKG2D expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with NKG2D Mouse mAb (Biotin) (Ab185179) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.5μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

KLRK1 Tbio NKG2-D type II integral membrane protein (0 Activities)

Discover Target: KLRK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)