GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human Cofilin 1 Antibody; Monoclonal antibody (C12), could be used for WB, IHC and so on. Application WB: 0.2-2µg/mL IHC: 5-20µg/mL IF/ICC: 5-30µg/mL Purity Protein A + Protein G affinity chromatography Function Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Specifications
Product Name
Cofilin 1 Mouse mAb - Primary antibody, specific to Cofilin-1, Mouse IgG1
Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species
Mouse
Specificity
Cofilin-1
Immunogen
Recombinant CFL1 (Ser3~Leu161) expressed in E.coli
Positive Control
WB: MCF7, A431, Hep G2, HeLa, HEK293 cell lysates. IHC: Human small intestine tissues, Human liver tissues, Human spleen tissues. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G affinity chromatography
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term. Aliquot and store at -20°C long term（24 months）. Avoid freeze/thaw cycles.
Images
Cofilin 1 Mouse mAb (Ab097098) - Western Blot All lanes: Cofilin 1 Mouse mAb (Ab097098) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 1.0 s
Cofilin 1 Mouse mAb (Ab097098) - IHC DAB staining on IHC Sample: Human Small intestine Tissue Primary Ab: 20µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2µg/mL HRP Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Cofilin 1 Mouse mAb (Ab097098) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Cofilin 1 Mouse mAb (Ab097098) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Cofilin 1 Mouse mAb (Ab097098) - IF/ICC AF488 staining on IF Sample: Human HeLa cell Primary Ab: 30 µg/mL Cofilin 1 Mouse mAb (Ab097098) Second Ab: 2 µg/mL AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Application
Application
Dilution info
IHC
5-20 µg/mL
WB
0.2-2 µg/mL
IF/ICC
5-30µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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