GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human IDH1 Antibody, Monoclonal antibody (C12), could be used for WB,IHC and so on. Application: WB: 0.01-2µg/mL IHC:5-30µg/mL Protein Function
Specifications
Product Name
IDH1 Mouse mAb - Primary antibody, specific to IDH1, Mouse IgG2a
Carrier Free, ExactAb™, Validated, High Performance, See COA
Host species
Mouse
Specificity
IDH1
Immunogen
Recombinant IDH1 expressed in E.coli (AA 74-333).
Positive Control
WB: Hep G2 and NIH/3T3 cell lysates. IHC: Human colorectal cancer, stomach, kidney, prostate, glioma tissues.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
IDH1 Mouse mAb (Ab109324) - Western Blot All lanes: IDH1 Mouse mAb (Ab109324) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/40000 dilution
Predicted band size: 47 kDa Observed band size: 44 kDa Exposure time: 25.4 s
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Colorectal cancer Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Stomach Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Prostate Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Glioma Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Prostate Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
IDH1 Mouse mAb (Ab109324) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 30 µg/mL IDH1 Mouse mAb (Ab109324) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Application
Application
Dilution info
IHC
5-30 µg/mL
WB
0.01-2 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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