ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti Human TIMP2 Antibody, Monoclonal (1554CT494.262.47), could be used for WB, IHC, ICC, IF, Flow and so on.
Application
WB: 1/2000
IHC: 1/200
IF: 1/25
Flow: 1/25
Protein Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
TIMP2 Mouse mAb (Ab131343) - Western Blot
All lanes: TIMP2 Mouse mAb (Ab131343) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 21 kDa
Exposure time: 120.0 s
TIMP2 Mouse mAb (Ab131343) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling TIMP2 with TIMP2 Mouse mAb (Ab131343) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
TIMP2 Mouse mAb (Ab131343) - IHC
Immunohistochemical analysis of paraffin-embedded Human kidney section using TIMP2 Mouse mAb (Ab131343). TIMP2 Mouse mAb (Ab131343) was diluted at 1/200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
TIMP2 Mouse mAb (Ab131343) - IHC
TIMP2 Mouse mAb (Ab131343) staining TIMP2 in human kidney sections by Immunohistochemistry. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with TIMP2 Mouse mAb (Ab131343) (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
TIMP2 Mouse mAb (Ab131343) - Flow Cytometry
Overlay histogram showing K562 cells stained with TIMP2 Mouse mAb (Ab131343) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the TIMP2 Mouse mAb (Ab131343) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.
| Application | Dilution info |
|---|---|
| Flow Cytometry | 1/25 |
| WB | 1/2000 |
| IHC | 1/200
|
| IF/ICC |
1/25
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102590 | Certificate of Analysis | Dec 16, 2025 | Ab131343 |
| ZJ24F0102591 | Certificate of Analysis | Dec 16, 2025 | Ab131343 |
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