DL-Dithiothreitol(DTT) - UltraBio™, Suitable for molecular biology, ≥99.5%(RT) , CAS No.3483-12-3

CAS: 3483-12-3 Cat. No.: D755747 Formula: C₄H₁₀O₂S₂ Molecular Weight: 154.25 Beilstein Registry Number: 1719760 EC Number: 222-468-7

GRADE & PURITY UltraBio™, Suitable for molecular biology, ≥99.5%(RT)

Synonyms

DTT | (±)-Dithiothreitol | rac-Dithiothreitol | Dithiothreitol | threo-1,4-Dimercapto-2,3-butanediol | Cleland’s reagent

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Dithiothreitol (DTT) is a thiol-reducing reagent that quantitatively reduces disulfide (SH) groups in biological molecules. It specifically targets the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is more efficient than that of 2-mercaptoethanol (5% v/v, 700 mM). 
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). 
DTT has been used:as an enzyme stabilizer to maintain exebacase stability. for peptide extraction and protein denaturation. in sample preparation and reversal of formaldehyde crosslinks before mass spectrometry. as a buffer component to extract synaptogliosome.

Specifications

Synonyms

DTT | (±)-Dithiothreitol | rac-Dithiothreitol | Dithiothreitol | threo-1,4-Dimercapto-2,3-butanediol | Cleland’s reagent

Specifications & Purity

UltraBio™, Suitable for molecular biology, ≥99.5%(RT)

Biochemical and Physiological Mechanisms

Dithiothreitol (DTT) is broadly used in chemical peptide synthesis and biochemical preparations of thiol proteins. Additionally, studies on protein folding and enzyme activity in protein chemistry make use of DTT. This reagent explicitly mediates the thio

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Suitable for molecular biology, UltraBio™

Purity

≥99.5%(RT)

Names and Identifiers

Pubchem Sid

528764159

PH

4.0-6.5 (25 °C, 0.1 M in H 2 O)

Canonical Smiles

O[C@H](CS)[C@H](O)CS

IUPAC Name

(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol

InChIKey

VHJLVAABSRFDPM-QWWZWVQMSA-N

INCHI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

Isomeric SMILES

C([C@H]([C@@H](CS)O)O)S

WGK Germany

3

RTECS

EK1610000

Alternate CAS

27565-41-9

Molecular Weight

154.25

Beilstein

1719760

Reaxy-Rn

9483598

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=9483598&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic oxygen compounds
    • Class Organooxygen compounds
      • Subclass Alcohols and polyols
        • Level5 Polyols
          • Level6 1,2-diols

• Kingdom: Organic compounds
• Superclass: Organic oxygen compounds
• Class: Organooxygen compounds
• Subclass: Alcohols and polyols
• Intermediate Tree Nodes: Polyols
• Direct Parent: 1,2-diols
• Alternative Parents: Secondary alcohols Alkylthiols Hydrocarbon derivatives
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Secondary alcohol - 1,2-diol - Alkylthiol - Hydrocarbon derivative - Organosulfur compound - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as 1,2-diols. These are polyols containing an alcohol group at two adjacent positions.
• External Descriptors: 1,4-dithiothreitol

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: H 2 O: 0.1 M at 20 °C, clear, colorless
• Flash Point(°F): 235.4 °F
• Flash Point(°C): 113 °C
• Boil Point(°C): 125～130°C
• Melt Point(°C): 42-43°C
• Molecular Weight: 154.300 g/mol
• XLogP3: -0.400
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 3
• Exact Mass: 154.012 Da
• Monoisotopic Mass: 154.012 Da
• Topological Polar Surface Area: 42.500 Ų
• Heavy Atom Count: 8
• Formal Charge: 0
• Complexity: 52.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 2
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1