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PhosphateBuffered Saline（PBS）20X concentrate,PH 7.5 - Ultra pure

Cat. No.: P274233
COA
PhosphateBuffered Saline（PBS）20X concentrate,PH 7.5 - Ultra pure
AVAILABLE TO ORDER
GRADE & PURITY Ultra pure ? Ultra-pure grade with very low impurity content across the board. Use for trace analysis, electronics, or processes intolerant of contamination.
Synonyms
PBS
Storage
Room temperature
Shipped In
Normal
★
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
500ml
P274233-500ml
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$74.90
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SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Overview Specifications Names and Identifiers Documentation Advanced Data Certificates（CoA,COO,BSE/TSE and Analysis Chart) Chemical and Physical Properties Documents & Articles Solution Calculators Reviews
🧪

Why this grade

Ultra pure Ultra pure for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Room temperature Ships Normal Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

1X Solution Composition:137 mM NaCl, 2.7 mM KCl, 9.5 mM Phosphate buffer Does not contain magnesium or calcium. Solution is 0.2 µm filtered into bottles and autoclaved. Ready-to-use as supplied. pH at 25°C (1X PBS): 7.1 – 7.7 DNase, RNase, Protease none detected. Suitable for cell culture.

Specifications

Synonyms
PBS
Specifications & Purity
Ultra pure
Storage
Room temperature
Shipped In
Normal
Grade
Ultra pure
Names and Identifiers
PH7.1–7.7

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

14 results found

Lot NumberCertificate TypeDateItem
C2620508Certificate of AnalysisMar 11, 2026 P274233
K2321423Certificate of AnalysisSep 04, 2025 P274233
G2508524Certificate of AnalysisJun 19, 2025 P274233
G2508525Certificate of AnalysisJun 12, 2025 P274233
C2528453Certificate of AnalysisMar 22, 2025 P274233
I2425580Certificate of AnalysisSep 18, 2024 P274233
I2425597Certificate of AnalysisSep 13, 2024 P274233
E2431317Certificate of AnalysisApr 23, 2024 P274233
C2419487Certificate of AnalysisJan 29, 2024 P274233
C2419488Certificate of AnalysisJan 29, 2024 P274233
I2301101Certificate of AnalysisAug 21, 2023 P274233
F23251465Certificate of AnalysisJun 15, 2023 P274233
B2224972Certificate of AnalysisJul 12, 2022 P274233
K2118993Certificate of AnalysisDec 13, 2021 P274233

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Chemical and Physical Properties
SolubilityFor making 1X solution dilute 1:20 in deionized water
Documents & Articles
Flow cytometry protocol
Categories: Protocols
Tags:
Antibodies Flow Cytometry Flow Antibodies Reactive dyes Indirect antibody labelling
Native ChIP (N-ChIP) Protocol
Categories: Protocols
Tags:
Antibody Biological buffer Native ChIP Protein Electrophoresis & Western Blotting Chromosome
Aladdin@Guidelines for the formulation of commonly used buffers for biological experiments
Categories: Technical articles
Tags:
Biochemical reagents Antibiotics Western blot Stains and dyes Electrophoresis Biological buffers
Cross-linking ChIP-Seq (X-ChIP-Seq) protocol
Categories: Protocols
Tags:
Electrophoresis Cross-linking ChIP
Chromatin preparation from tissues for chromatin immunoprecipitation (ChIP)
Categories: Protocols
Tags:
Electrophoresis ChIP Lysis buffer Aprotinin
Experimental Guide of Sample Preparation for Flow Cytometry
Categories: Protocols
Tags:
Protein sample preparation Flow Cytometry Antibody FACS
Immunohistochemical staining protocol for frozen samples (IHC-Fr)
Categories: Protocols
Tags:
IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies
RNA dot blot protocol
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies
GST Pull-Down Protocol
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting GST Pull-Down Protein-protein interactions
In-cell ELISA protocol
Categories: Protocols
Tags:
Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies
Enzyme-linked Immunospot Technique ( ELISPOT ) Protocol
Categories: Protocols
Tags:
Cytokines Growth factors ELISPOT Secreted Proteins HRP Conjugated Secondary Antibodies
Immunopeptide blocking assay
Categories: Protocols
Tags:
Immunopeptideblocking PolyclonalAntibody SpecificBinding
BrdU staining protocol
Categories: Protocols
Tags:
Nucleosides & nucleotides Cell Proliferation Cell Division Nucleoside Analogues BRDU Protein Electrophoresis Gels & Buffers Flow Cytometry Antibodies
Caspase Immunofluorescence Staining Protocol
Categories: Protocols
Tags:
Secondary antibody Apoptosis Programmed Death Immunofluorescence Caspase
BMDC Isolation Method
Categories: Protocols
Tags:
Cytokines GM-CSF BMDC Dendritic cells DC Bone marrow M-CSF RPMI
General Protocol for Western Blotting
Categories: Protocols
Tags:
Western blotting WB Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Conjugated Secondary Antibodies HRP
Methods of Lysate Preparation for Western Blotting
Categories: Protocols
Tags:
Western blotting SDS Cell lysis Tissue dissociation Protein extraction RIPA buffer
Western Blotting with Fluorescently Labeled Antibodies
Categories: Protocols
Tags:
Protein Electrophoresis & Western Blotting Biological buffers Immunoblotting Fluorescently Labeled Antibodies Membrane Transfer
BN-PAGE Protocol
Categories: Protocols
Tags:
Western blotting Natural polypropylene gel electrophoresis Non-denaturating gel electrophoresis BN-PAGE Coomasil brilliant blue Protein complex Horseradish peroxidase Alkaline phosphatase
Histone Western Blot
Categories: Protocols
Tags:
Monoclonal antibodies Western blot Recombinant Antibodies Immunoblotting HRP Horseradish peroxidase Histone
Protocol of Western blot for Phosphorylated Proteins
Categories: Protocols
Tags:
Western blot ECL Phosphorylated proteins Phosphorylated Antibodies Immunoblot HRP-conjugated Antibodies
Protein Dephosphorylation Protocol
Categories: Protocols
Tags:
SDS-PAGE Alkaline phosphatase Phosphorylated Antibodies Protein Electrophoresis AP CIP Dephosphorylation Calf intestinal alkaline phosphatase Transfer
In Situ Hybridization (ISH) Protocol
Categories: Protocols
Tags:
protease Paraffin Section Frozen section ISH In situ hybridization RNA probe DNA probe Digoxigenin labeling
Enzyme Protectants and Common Methods to Prolong Enzymatic Activity
Categories: Technical articles
Tags:
Enzymes Biocatalysts Enzyme Protectants
Buffer exchange of a sample using a dialysis bag
Categories: Protocols
Tags:
Dialysis bag Dialysis
β-Propiolactone (BPL) Viral Inactivation — Standard Operating Procedure (SOP)
Categories: Protocols
Tags:
β-Propiolactone BPL
Red Blood Cell Lysis Buffer — Technical Description & Operating Guide
Categories: Protocols
Tags:
Red Blood Cell Lysis Buffer
Professional Guide to Streptavidin-Modified Proteins
Categories: Technical articles
Tags:
Avidin Streptavidin
Common Reducing Agents Used in Protein Experiments
Categories: Technical articles
Tags:
Reducing agents Protein Experiments
Common Reagents for Reducing Protein Disulfide Bonds
Categories: Technical articles
Tags:
Reducible protein Protein disulfide bond
Common Protectants and Formulations for Protein Storage
Categories: Technical articles
Tags:
Protein storage
Why Did Nickel Agarose Beads Lose Their Color?
Categories: Technical articles
Tags:
Nickel Agarose Beads NTA HTC
Purifying Antibodies with Magnetic Agarose Beads
Categories: Protocols
Tags:
Antibody Purification Magnetic agarose microspheres
Hoechst Staining for Apoptosis Detection: Experimental Workflow
Categories: Protocols
Tags:
Fluorescent dyes Hoechst
EdU Staining: Basic Principle and Experimental Workflow
Categories: Protocols
Tags:
Thymidine nucleoside EdU staining
HepG2 Cell Steatosis Model Establishment Protocol
Categories: Protocols
Tags:
HepG2 Human liver cancer cell line
Isolation, Culture, and Induced Differentiation of Bone Marrow Mesenchymal Stem Cells into Osteoclasts: Experimental Procedure
Categories: Protocols
Tags:
Bone Marrow Mesenchymal Stem Cells
FeRhoNox-1 (Fe²⁺ Indicator) Fluorescent Probe Instruction Manual — Practical Guide for Specific Detection of Intracellular Fe²⁺ in Live Cells
Categories: Technical articles
Tags:
FeRhoNox-1 Live cell fluorescent probe
Differences Among Antibodies for WB/FC/IP/ChIP/IF/IHC
Categories: Technical articles
Tags:
Antibodies Protein Research
GST-Tagged Protein Purification
Categories: Protocols
Tags:
Protein purification Glutathione S-transferase GST-Tagged Protein Purification GST
Biotin-Labeled Protein Purification
Categories: Protocols
Tags:
Biotin-Labeled Protein Purification Biotinylation
Hyaluronidase: Unlocking a “Magic Enzyme” in Biomedicine
Categories: Technical articles
Tags:
Enzymes Hyaluronic acid
Scientific Decision-Making for Chromatography Buffer Selection: Tris-HCl vs Phosphate Systems
Categories: Technical articles
Tags:
Buffer Chromatography
RNA Pull-Down Standard Operating Procedure (SOP)
Categories: Protocols
Tags:
RNA Pull-Down
DNA Pull-Down Standard Operating Procedure (SOP)
Categories: Protocols
Tags:
DNA Pull-Down
CCK-8 (WST-8) Colorimetric Cell Viability Assay: Principles, Experimental Design, and Typical Applications
Categories: Technical articles
Tags:
Cell Proliferation Cytotoxicity CCK-8 WST-8 cell viability drug sensitivity
16 Frequently Asked Questions About Nourseothricin
Categories: FAQs
Tags:
Nourseothricin
Selection Strategy for PBS, TBS, PBST, and TBST Washing Systems in IHC
Categories: Technical articles
Tags:
PbS IHC Tween 20 TBS PBST TBST HRP-DAB AP chromogenic detection washing system
Comparison of Principles, System Control, and Result Interpretation in Hydroxyl Radical Scavenging Capacity Assays
Categories: Technical articles
Tags:
Antioxidant Evaluation Chemiluminescence Hydroxyl Radical Scavenging Capacity Fenton Reaction Salicylic Acid Method Deoxyribose Assay Electron Spin Resonance
Avertin Anesthetic (Avertin, 2,2,2-Tribromoethanol): Overview, Applications, and Experimental Precautions
Categories: Technical articles
Tags:
Quality control Avertin tribromoethanol mouse anesthesi intraperitoneal injection peri-anesthetic management adverse reactions
Solution Calculators
Reviews

Customer Reviews

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